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In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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Summary
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Cystic fibrosis transmembrane conductance regulator (CFTR), an epithelial chloride channel, has been reported to interact with various proteins and regulate important cellular processes; among them the CFTR PDZ motif-mediated interactions have been well documented. This protocol describes methods we developed to assemble a PDZ-dependent CFTR macromolecular signaling complex in vitro.

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In Vitro Analysis PDZ-dependent CFTR Macromolecular Signaling Complexes CFTR Chloride Channel Transepithelial Fluid Homeostasis Cystic Fibrosis Secretory Diarrhea CFTR Cl- Channel Physical Interactions Functional Interactions Transporters Ion Channels Receptors Kinases Phosphatases Signaling Molecules Cytoskeletal Elements CFTR Carboxyl Terminal Tail PSD95/Dlg1/ZO-1 (PDZ) Motif Scaffold Proteins
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