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Neuroscience

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JoVE Journal Neuroscience
Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
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Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Article DOI: 10.3791/4163-v • 06:31 min • July 26th, 2012
July 26th, 2012 •
1, 1, 1, 1, 2, 3, 1
1Division of Molecular Neurobiology, MRC National Institute for Medical Research, 2Confocal and Image Analysis Laboratory, National Institute for Medical Research, 3Physiopathologie de la plasticité neuronale, Neurocentre Magendie, Université de Bordeaux

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Summary
Automatic Translation

This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.

Tags

Visualization Genetic Manipulation Dendrites Spines Mouse Cerebral Cortex Hippocampus In Utero Electroporation IUE Development Nervous System Cellular Proliferation Neuronal Migration Cerebral Cortex Hippocampus Neurons Primary Cultures Dendrite Development Spine Development Synapse Development Golgi-Cox Method Fluorescent Constructs
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