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Immunology and Infection

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En ny Screening Method for Regissert Evolution of termostabilt bacteriolytic Enzymer

Article DOI: 10.3791/4216-v • 13:30 min • November 7th, 2012
November 7th, 2012 •
1, 1
1Institute for Bioscience and Biotechnology Research, University of Maryland

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En roman rettet evolusjon metode spesifikk til feltet av thermostability engineering ble utviklet og følgelig validert for bacteriolytic enzymer. Etter bare en runde av tilfeldig mutagenese, en utviklet bacteriolytic enzym, PlyC 29C3, vises større enn to ganger restaktivitet når sammenlignet med villtype-protein etter forhøyet temperatur inkubasjon.

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Immunologi Molecular Biology genetikk mikrobiologi regissert evolusjon termisk oppførsel thermostability endolysin enzybiotic bacteriolytic antimikrobiell terapeutiske PlyC
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