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An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
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An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

Article DOI: 10.3791/4245-v • 07:45 min • January 20th, 2013
January 20th, 2013 •
1, 1
1Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center

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Summary
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The cranial mesenchyme undergoes dramatic morphogenic movements that likely provides a driving force for elevation of the neural folds1,2. Here we describe a simple ex vivo explant assay to characterize the cellular behaviors of the cranial mesenchyme during neurulation. This assay has numerous applications including being amenable to pharmacological manipulations and live imaging analyses.

Tags

Explant Assay Cellular Behavior Cranial Mesenchyme Central Nervous System Neural Plate Neurulation Morphogenetic Movements Neural Tube Formation Paraxial Mesoderm Neural Crest Cells Porous Meshwork Stellate Shaped Cells Extracellular Matrix (ECM) Strands Stereotypical Rearrangements Expansion Driving Force Pathways Cellular Behaviors Interactions Between ECM And Cells Ex Vivo Assay Pharmacological Manipulations Signaling Pathways Live Imaging Analyses Migratory Properties
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