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Journal / Immunology and Infection / ペプチドエピトープ特異的T細胞のMHCテトラマーベースの濃縮…
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Immunology and Infection

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JoVE Journal Immunology and Infection
ペプチドエピトープ特異的T細胞のMHCテトラマーベースの濃縮
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Journal (Immunology and Infection)
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
Journal (Immunology and Infection)
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
 
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ペプチドエピトープ特異的T細胞のMHCテトラマーベースの濃縮

Article DOI: 10.3791/4420-v • 13:58 min • October 22nd, 2012
October 22nd, 2012 •
1, 1
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

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エピトープ特異的T細胞の低周波の集団を区別し、フローサイトメトリーによって、それらを分析するためのMHCテトラマーと磁性マイクロビーズ：このプロトコルは、ペプチドの使用について説明します。この方法は、から対象の内因性T細胞集団の直接調査を可能に

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免疫学、問題68、細胞生物学、分子生物学、T細胞は、T細胞受容体、四量体、フローサイトメトリー、抗原特異的、免疫学、免疫応答、磁気、濃縮、
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