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Journal / Immunology and Infection / Peptid: MHC Tetramer-baserad Anrikning av…
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Immunology and Infection

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Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Journal (Immunology and Infection)
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Peptid: MHC Tetramer-baserad Anrikning av Epitop-specifika T-celler

Article DOI: 10.3791/4420-v • 13:58 min • October 22nd, 2012
October 22nd, 2012 •
1, 1
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

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Detta protokoll beskriver användningen av peptid: MHC tetramerer och magnetiska mikropärlor att isolera lågfrekventa populationer av epitop-specifika T-celler och analysera dem genom flödescytometri. Denna metod möjliggör direkta studier av endogena T-cell populationer av intresse från

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Immunologi 68 Cellulär Biology molekylärbiologi T-cell T-cellreceptor tetramer flödescytometri antigenspecifik immunologi immunsvar magnetisk anrikning,
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