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JoVE Journal Neuroscience
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
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Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Article DOI: 10.3791/50025-v • 11:24 min • December 12th, 2012
December 12th, 2012 •
1, 1, 1, 1, 1, 1
1Department of Neuroscience, Medical University of South Carolina

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Summary
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A method is described for labeling neurons with fluorescent dyes in predetermined functional micro-domains of the neocortex. First, intrinsic signal optical imaging is used to obtain a functional map. Then two-photon microscopy is used to label and image neurons within a micro-domain of the map.

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Neurons Labeling Functional Micro-domain Neocortex Intrinsic Signal Imaging Two-photon Imaging Primary Visual Cortex Stimulus Features Orientation Selectivity Continuous Map Preferred Orientation Synaptic Input Dendritic Spines Neuronal Cell Bodies Functional Maps Calcium Sensitive Dyes
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