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JoVE Journal Immunology and Infection
Kvantitativ Højkapacitetsforskning encellede cytotoksicitetsassay for T-celler
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Kvantitativ Højkapacitetsforskning encellede cytotoksicitetsassay for T-celler

Article DOI: 10.3791/50058-v • 09:28 min • February 2nd, 2013
February 2nd, 2013 •
1, 2, 1, 2, 1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

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Vi beskriver en enkelt-celle high-throughput assay til måling af cytotoksicitet af T-celler, når inkuberet med tumormålceller. Denne fremgangsmåde anvender en tæt, elastomere matrix af sub-nanoliter brønde (~ 100.000 brønde / række) til rumligt begrænse de T-celler og målceller ved definerede forhold og er koblet til fluorescensmikroskopi for at overvåge effektor-mål-konjugation og efterfølgende apoptose.

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Cancer Biology Immunology Cellular Biology molekylærbiologi medicin Kemiteknik Biomolecular Engineering Bioengineering Immunterapi Adoptiv Microfluidics Nanowell arrays PDMS BioStation T-celler tumormålceller mærkning cytotoksicitet mikroskopi assay
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