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JoVE Journal Bioengineering
Kvantifisere mekaniske egenskaper endotel Glycocalyx med Atomic Force Microscopy
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Kvantifisere mekaniske egenskaper endotel Glycocalyx med Atomic Force Microscopy

Article DOI: 10.3791/50163-v • 10:24 min • February 21st, 2013
February 21st, 2013 •
1, 1
1Department of Biomedical Engineering, University of Rochester

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De mekaniske egenskapene til endotelial glycocalyx ble målt ved hjelp innrykk mikrometerstørrelse sfærer på AFM cantilevers. Endotelceller ble dyrket i en tilpasset kammer under fysiologiske strømningsforhold for å indusere glycocalyx uttrykk. Data ble analysert ved hjelp av en tynn film for å fastslå den glycocalyx tykkelse og modulus.

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Biomedical Engineering bioteknologi cellebiologi biofysikk Molecular Biology Endotelet Vaskulær membran glykoproteiner Reseptorer Leukocytt-Adhesion bioteknologi (generelt) glycocalyx mekaniske egenskaper atomic force mikroskopi ATM endotelceller leukocytter cellevegg cellekultur mikroskopi bildebehandling
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