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Purification of Transcripts and Metabolites from Drosophila Heads
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Purification of Transcripts and Metabolites from Drosophila Heads

Article DOI: 10.3791/50245-v • 12:49 min • March 15th, 2013
March 15th, 2013 •
1, 1, 2, 1, 1, 3, 2, 3, 1,4, 1,4
1Department of Neurology, McKnight Brain Institute, University of Florida, 2Department of Entomology and Nematology, University of Florida, 3Genetics Institute, Department of Molecular Genetics and Microbiology, University of Florida, 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration, University of Florida

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Summary
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We describe here the procedures for the extraction and purification of mRNA and metabolites from Drosophila heads. We are applying these techniques to better understand the cellular perturbations underlying neuronal degeneration. These methodologies can be easily scaled and adapted for other "omic" projects.

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Purification Transcripts Metabolites Drosophila Heads Neuronal Degeneration Model Organism Neurodegenerative Diseases Traditional Techniques Genetic Interaction Histology Immunofluorescence Protein Biochemistry Omics Age Cellular Perturbations Complex Diseases Model Organisms DNA MRNA Protein Metabolites Fly Brains Experimental Replicates Biological Material
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