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Vorbereitung des Mgm101 Rekombinationsprotein von MBP-based Tagging-Strategie
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Vorbereitung des Mgm101 Rekombinationsprotein von MBP-based Tagging-Strategie

Article DOI: 10.3791/50448-v • 11:40 min • June 25th, 2013
June 25th, 2013 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

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Die Hefe-Mitochondrien nucleoid Protein, Mgm101, ist ein Rad52-Typ Rekombinationsprotein die große oligomere Ringe bildet. Ein Protokoll wird beschrieben, dass lösliches rekombinantes Mgm101 vorzubereiten mit dem Maltose Binding Protein (MBP)-Tagging-Strategie mit Kationenaustausch und Größenausschlusschromatographie gekoppelt.

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Biochemie Genetik Molekularbiologie Proteine ​​Mgm101 Rad52 Mitochondrien Rekombination mtDNA Maltose-bindende Protein
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