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Biochemical Titration of Glycogen In vitro
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Biochemical Titration of Glycogen In vitro

Article DOI: 10.3791/50465-v • 07:16 min • November 24th, 2013
November 24th, 2013 •
1, 1, 1, 1
1Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 - INSERM, U1081 - UNS, Centre Antoine Lacassagne, University of Nice - Sophia Antipolis

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Summary
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We describe here an accurate, reproducible and convenient biochemical method for titration of glycogen in vitro. This technique uses the Abcam Glycogen assay kit and is based on successive hydrolysis of glycogen to glucose and glucose titration by fluorescence.

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Biochemical Titration Glycogen In Vitro Energetic Polymer Glucose Vertebrate Animals Whole Body Metabolism Cellular Metabolism Detection Methods Quantitative Abcam Glycogen Assay Kit Glucoamylase Glucose Degradation OxiRed Probe Fluorescence Accurate Sensitive Cell Extracts Tissue Sections Distribution Of Glycogen Chinese Hamster Lung Fibroblast CCL39 Human Colon Carcinoma LS174 Normoxia Hypoxia Signal Synthesize Glycogen
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