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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

Article DOI: 10.3791/50531-v • 18:25 min • August 25th, 2013
August 25th, 2013 •
1, 1, 1
1Department of Cell Biology and Molecular Genetics, University of Maryland

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Live imaging of cells exposed to the lipophilic dye FM1-43 allows precise determination of the kinetics by which pore-forming toxins are removed from the plasma membrane. This is a sensitive assay that can be used to assess requirements for Ca2+, sphingomyelinase and other factors on plasma membrane repair.

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Live Imaging Assay Assessing Roles Ca2+ Sphingomyelinase Repair Pore-forming Toxin Wounds Plasma Membrane Injury Cellular Survival Ca2+ Influx Signaling Event Mechanical Wounds Lysosomal Enzyme Acid Sphingomyelinase Pore Endocytosis Toxin Streptolysin O Assay Design Injury Event Synchronization Kinetic Measurement Plasma Membrane Integrity Restoration Liphophilic Dye FM1-43 Intracellular Membranes Exogenously Added Soluble Factors Sphingomyelinase Inhibition First Time Discovery
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