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Protein Purification-fri Metod för bindningsaffinitet Bestämning genom Microscale Thermophoresis
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Protein Purification-fri Metod för bindningsaffinitet Bestämning genom Microscale Thermophoresis

Article DOI: 10.3791/50541-v • 10:22 min • August 15th, 2013
August 15th, 2013 •
1,2, 1, 3, 4, 1, 1, 1
1Cancer and Inflammation Program, National Cancer Institute, 2Basic Science Program, SAIC-Frederick, Inc., 3Departments of Oncology and Radiation Medicine, Georgetown University Medical Center, 4Structural Biophysics Laboratory, National Cancer Institute

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Microscale thermophoresis (MST) kan ofta används för bestämning av bindningsaffinitet utan rening av målproteinet från cellysat. Protokollet involverar överuttryck av GFP-fuserade proteinet, cellys i icke-denaturerande betingelser, och detektion av MST-signal i närvaro av varierande koncentrationer av liganden.

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Molecular Biology Biochemistry Cellulär Biology genetik kemi farmakologi intracellulära signalerande peptider och proteiner proteiner protein-inhibitor interaktion K Transkriptionsfaktor ligandbindning bindningsaffinitet thermophoresis fluorescens mikroskopi
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