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Journal / Immunology and Infection / En Tetracyclin-regulert Cell Linje Produserer Høy …
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Immunology and Infection

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JoVE Journal Immunology and Infection
En Tetracyclin-regulert Cell Linje Produserer Høy titer lentiviral vektorer som spesifikt retter dendrittiske celler
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Journal (Immunology and Infection)
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Journal (Immunology and Infection)
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En Tetracyclin-regulert Cell Linje Produserer Høy titer lentiviral vektorer som spesifikt retter dendrittiske celler

Article DOI: 10.3791/50606-v • 16:04 min • June 19th, 2013
June 19th, 2013 •
1, 1, 1, 1
1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles

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Her anvendes retroviral transduksjon og concatemeric transfeksjon for å skape en cellelinje som kan uttrykke komponentene i en lentiviral vektor (LV) i fravær av tetracyklin. Dette LV koder GFP og er pseudotyped med et glykoprotein, SVGmu, som er spesifikk for en reseptor på dendrittiske celler.

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Immunologi virologi genetikk molekylærbiologi cellebiologi biokjemi kjemi bioteknologi Biomedical Engineering medisin Infeksjon farmakologi Lentivirus kreftvaksiner vaksiner viruslignende partikkel biovitenskap mikrobiologi bioteknologi ( generelt) Lentiviral vektor stabil cellelinje dendrittiske celler vaksine concatemeric transfeksjon retrovirus virus plasmid cellekultur
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