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JoVE Journal Bioengineering
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
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Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level

Article DOI: 10.3791/50616-v • 11:49 min • November 17th, 2013
November 17th, 2013 •
1, 2,3, 1, 1, 1, 3, 2,3
1Department of Mechanical Engineering, University of Michigan-Dearborn, 2Department of Surgery/Transplant, University of Illinois at Chicago, 3Department of Bioengineering, University of Illinois at Chicago

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Summary
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Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.

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Quantitative Control Temporal Control Oxygen Microenvironment Single Islet Level Glucose Stimulus-secretion Coupling Factors Islet Hypoxia Transplant Environments Hypoxic Chamber Techniques Real-time Monitoring Multilayered Microfluidic Technique Aqueous And Gas Phase Modulations Diffusion Membrane Stimulation Sandwich Polydimethylsiloxane (PDMS) Device Fluorescence Microscopy Gas Input Control Microdispensers Quantitative Sub-minute Modulations Intermittent Hypoxia Islet Preconditioning Multimodal Microscopy Calcium Dynamics KATP Channel Dynamics Microfluidic Hypoxia
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