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JoVE Journal
Biology

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Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
 

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Article DOI: 10.3791/50684-v 07:11 min October 8th, 2013
October 8th, 2013

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Summary

Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.

Tags

Electrocompetent Escherichia Coli Vibrio Cholerae Electroporation DNA Introduction Prokaryotes Electrotransformation Gene Pulse Controller Cuvettes Transformation Method Centrifugation Washes Growth Phase Electrocompetent Cells Commercial Sources Laboratory Strains Bacteriology Laboratories
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