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JoVE Journal
Biology

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由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本
 
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由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本

Article DOI: 10.3791/50710-v 11:02 min October 18th, 2013
October 18th, 2013

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Summary

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Click here for the English version.

我们描述条形码DNA文库和随后的杂交为基础的外显子捕获检测的关键癌症相关的基因突变在临床肿瘤标本通过大规模并行的“下一代”测序的准备。靶向外显子的测序提供了高吞吐量,低成本,以及深的序列覆盖率的好处,从而产生较高的灵敏度,用于检测低频率突变。

Tags

分子生物学,80期,分子诊断技术,高通量核苷酸序列分析,遗传学,肿瘤,诊断,大规模并行测序,外显子靶向测序,杂交捕获,癌症,FFPE,DNA突变
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