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Western Blotting Protocol - ADVERTISEMENT

Minh-Ha Lieu1, Troy Humphreys1, Elisabeth Antanavich1, Susan Chow1
1Cell Signaling Technology, Inc.

Abstract

The western blotting technique measures protein expression in a cell or tissue extract using antibody-based detection of a target protein. The antibodies used in a western blot can be specific to a total protein or to unique post-translational modifications of a protein, such as sites of phosphorylation, acetylation, methylation, or ubiquitination. The broad range of available total protein and modification-specific antibodies allows researchers to study cellular signaling events involved in many biological contexts and processes. Here we provide a comprehensive western blot protocol developed and used at Cell Signaling Technology that includes optimal reagents and support information to ensure optimal results.

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