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Chemistry

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
 

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Article DOI: 10.3791/50747-v 14:44 min December 16th, 2013
December 16th, 2013

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Summary

The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

Tags

Peptide-metal Complexes 1D NMR 2D NMR Cu (I) Binding Metallochaperone Transport Proteins Copper Oxidation Redox Reactions Cu (I) Complex NMR Spectroscopy Solution Structures Proteins Peptides Crystallization X-ray Crystallography NMR Technique Sample Preparation Data Collection Peak Assignment Integration Molecular Mechanics Calculations Structure Analysis
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