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Neuroscience

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Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
 

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Article DOI: 10.3791/50782-v 11:34 min December 24th, 2013
December 24th, 2013

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Summary

Afferent sensory neurons signal sensory information from the periphery to the central nervous system. Identifying specific afferent neurons will help in understanding their physiology. We describe a method of retrograde labeling to identify afferent neurons, and study the voltage-gated ion channels in these neurons using patch clamp electrophysiology and immunocytochemistry.

Tags

Sensory Neurons Ion Channels Specific Neuron Populations Central Nervous System Dorsal Root Ganglia Receptors Channels Afferent Neurons Retrograde Labeling Fluorescent Dye DiI Excitation Muscle Afferents Ion Channel Distribution Pattern Patch Clamp Electrophysiology Immunocytochemistry
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