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Biology

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Single-cell Microinjection for Cell Communication Analysis
 

Single-cell Microinjection for Cell Communication Analysis

Article DOI: 10.3791/50836-v 10:00 min February 26th, 2017
February 26th, 2017

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Summary

We describe here how to perform a single-cell microinjection of Lucifer Yellow to visualize cellular communication via gap-junctions in living cells, and provide some useful tips. We expect that this paper will help everyone to evaluate the degree of cellular coupling due to functional gap junctions. Everything described here could be, in principle, adapted to other fluorescent dyes with molecular weight below 1,000 Daltons.

Tags

Single-cell Microinjection Cell Communication Analysis P2 Receptors Gap Injection Immune System Liver Fluorescence Microscope CCD Camera Current Generator Microelectrode Micro-manipulator Borosilicate Glass Capillary Polar Equipment Tungsten Filament Dye
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