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JoVE Journal Neuroscience
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Article DOI: 10.3791/50883-v • 10:30 min • January 12th, 2014
January 12th, 2014 •
*1, *1, 1, 1
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon
* These authors contributed equally

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Summary
Automatic Translation

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

Tags

PHluorin Axon Guidance Receptors Cell Culture Chick Embryo Development Attractive Cues Repulsive Cues Signaling Mechanisms Growth Cones Ligands Cell Surface Dynamics Spatio-temporal Visualization In Vitro Validation In Vivo Assessment
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