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Journal / Neuroscience / Nucleofection of Rodent Neuroblasts to Study…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Summary
Automatic Translation

Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

Tags

Nucleofection Rodent Neuroblasts Neuroblast Migration In Vitro Subventricular Zone (SVZ) Lateral Ventricles Adult Neurogenesis Neural Stem Cells Rostral Migratory Stream (RMS) Olfactory Bulb (OB) Radial Migration Mature Neurons Neurogenesis Functional Maturation Newborn Neurons Intracellular Mechanisms Motility Neuroregenerative Strategies Transfection Primary Rodent RMS Postnatal Neuroblasts 3D In Vitro Migration Assay Plasmid DNA Small Hairpin (sh)RNA Short Interfering (si)RNA Oligos
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