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JoVE Journal
Immunology and Infection

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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
 

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Article DOI: 10.3791/51033-v 09:33 min August 23rd, 2013
August 23rd, 2013

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Summary

Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.

Tags

VIGS-mediated Forward Genetics Screening Nonhost Resistance Plant Defense Genes Virus-induced Gene Silencing TRV-based VIGS Vector CDNA Library Nicotiana Benthamiana Nonhost Pathogens Pseudomonas Syringae Pv. Tomato T1 P. Syringae Pv. Glycinea X. Campestris Pv. Vesicatoria GFPuv Protein Gene Silenced Plants Nonhost Pathogen Inoculated Plants Target Gene Sequencing
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