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JoVE Journal Bioengineering
Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Article DOI: 10.3791/51102-v • 11:17 min • February 10th, 2014
February 10th, 2014 •
1, 1, 2, 1, 1
1Department of Bioelectronics, Ecole Nationale Superieure des Mines, 2Research and Exploratory Development Division, Applied Physics Laboratory, Johns Hopkins University

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Summary
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The Organic Electrochemical Transistor is integrated with live cells and used to monitor ion flux across the gastrointestinal epithelial barrier. In this study, an increase in ion flux, related to disruption of tight junctions, induced by the presence of the calcium chelator EGTA (ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid), is measured.

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Barrier Tissue Gastrointestinal Tract Pathogens Toxins Extracellular Calcium Concentration Conformational Change Paracellular Flux Calcium Chelator Ethylene Glycol-bis(beta-aminoethyl Ether)-N,N,N& 39;',N& 39;-tetra Acetic Acid (EGTA) Monolayer Of Cells Disruption Detection Methods Immunofluorescence Permeability Assays Electronic Methods Transepithelial Resistance (TER) Rapid Methods Cheap Methods Sensitive Methods Drug Discovery Pathogen/toxin Diagnostics Organic Electrochemical Transistor (OECT)
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