Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
 

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Article DOI: 10.3791/51215-v 10:13 min June 3rd, 2014
June 3rd, 2014

Chapters

Summary

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

Tags

Microfluidic Platform Measuring Neutrophil Chemotaxis Unprocessed Whole Blood Neutrophil Counts Infections Neutrophil Migration Microfluidic Assay Chemoattractant Red Blood Cells Mechanical Filters
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter