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Neuroscience

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JoVE Journal Neuroscience
A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
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A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Article DOI: 10.3791/51219-v • 09:37 min • June 15th, 2014
June 15th, 2014 •
1, 1, 1
1Regenerative Medicine Institute, Cedars-Sinai Medical Center

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Summary
Automatic Translation

This protocol describes a novel mechanical chopping method that allows the expansion of spherical neural stem and progenitor cell aggregates without dissociation to a single cell suspension.  Maintaining cell/cell contact allows rapid and stable growth for over 40 passages.

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CGMP Expansion Method Aggregates Human Neural Stem Cells Progenitor Cells Pluripotent Stem Cells Fetal Brain Tissue Research Experiments Clinical Trials Cell Expansion Technique Laborious Costly Complete Dissociation Differentiation Early Senescence Automated Mechanical Passaging Method Chopping Method Suspended Cultures Spheroid Cultures Constant Cell/cell Contact Neurospheres Embryonic Stem Cells Induced Pluripotent Stem Cells Tissue Culture Petri Dish Sterile Blade Automating Process
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