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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Article DOI: 10.3791/51228-v • 10:39 min • September 14th, 2014
September 14th, 2014 •
1, 1, 1, 1, 1, 1, 1, 1
1Department of Structural Biology, Max Planck Institute of Biophysics

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We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.

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Electron Cryo-tomography ATP Synthase Dimers Mitochondria Structural Biology Three-dimensional Structure Biological Samples Liquid Ethane Frozen-hydrated State Electron Microscope Tilt Series 3D Tomograms Subtomogram Averaging Noise Reduction 3D Maps Resolution Atomic Models Protein Complexes Native Environment Membrane Protein Complexes Inner Membrane Cristae
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