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In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
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In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

Article DOI: 10.3791/51229-v • 05:24 min • March 22nd, 2014
March 22nd, 2014 •
1, 1, 1
1Institute for Pharmacology, University of Heidelberg

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This report describes a simple and rapid technique of intrathecal needle puncture for a localized transfection of siRNA in the lumbar spinal cord in mouse under short lasting light anesthesia.

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In Vivo SiRNA Transfection Gene Knockdown Spinal Cord Rapid Noninvasive Lumbar Intrathecal Injections Mice Technique Acute Intrathecal Needle Injections Noninvasive Catheter Implantation Surgical Technique Hands-on Finesse Tissue Disruption Repeated Injections Immune Reaction Foreign Tools Specific Readout Spinal Cord Modulation Target Region Small Dosages Systemic Delivery Circumvented In Vivo Transfection Nucleic Acid Delivery Polyethylenimine (PEI) Reagent
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