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Glicopeptide Capture per Proteomica cellulari di superficie
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Glicopeptide Capture per Proteomica cellulari di superficie

Article DOI: 10.3791/51349-v • 10:11 min • May 9th, 2014
May 9th, 2014 •
1, 1
1Department of Chemistry, Simon Fraser University

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Proteine ​​di superficie cellulare sono biologicamente importante e ampiamente glicosilata. Vi presentiamo qui un approccio glicopeptide-capture per solubilizzare, arricchire, e deglycosylate queste proteine ​​per facili analisi proteomica basati LC-MS.

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Biologia Molecolare Numero 87 proteina di membrana glicoproteina N-linked modificazione post-traslazionale spettrometria di massa HPLC chimica Idrazide N-glycoproteomics cattura glicopeptide
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