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JoVE Journal Bioengineering
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
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Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Article DOI: 10.3791/51510-v • 08:35 min • February 21st, 2014
February 21st, 2014 •
1, 1, 1, 3, 4,5, 1,2
1Department of Mechanical Engineering, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 4Department of Bioengineering, University of California, Berkeley, 5Physical Biosciences Division, Lawrence Berkeley National Laboratory

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Summary
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Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.

Tags

Lipid Bilayer Vesicle Microfluidic Jetting Bottom-up Synthetic Biology Biochemical Systems Minimal Organisms Artificial Cells Giant Unilamellar Vesicles (GUVs) Model Platform Membrane Structure Microjetting Technique Controlled Size Membrane Composition Transmembrane Protein Incorporation Encapsulation Piezo-actuated Inkjet Device Soap Bubbles Customized Vesicles Droplet Interface Bilayer
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