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Immunology and Infection

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JoVE Journal Immunology and Infection
La detección in situ de las células T CD4 Autoreactive en Cerebro y Corazón Uso Mayor de Histocompatibilidad clase II Dextramers
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La detección in situ de las células T CD4 Autoreactive en Cerebro y Corazón Uso Mayor de Histocompatibilidad clase II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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El protocolo para detectar células autorreactivas T CD4 en el cerebro y el corazón mediante tinción directa con complejo mayor de histocompatibilidad de clase II dextramers se ha descrito en el presente informe. Para el análisis integral, un método fiable para enumerar las frecuencias de células T CD4 + específicas de antígeno in situ también se ideó.

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Inmunología Número 90 dextramers; MHC de clase II;
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