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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ Påvisning av autoreaktive CD4 T-celler i hjernen og hjertet Bruke store histocompatibility komplekse klasse II Dextramers
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In Situ Påvisning av autoreaktive CD4 T-celler i hjernen og hjertet Bruke store histocompatibility komplekse klasse II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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Protokollen for å oppdage selvreaktive CD4 T-celler i hjernen og hjertet ved direkte farging med store histocompatibility komplekse klasse II dextramers har blitt beskrevet i denne rapporten. For fullstendig analyse, er en pålitelig metode for å nummerere frekvensene av antigen-spesifikke CD4 + T-celler in situ også utviklet.

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Immunologi dextramers; MHC klasse II;
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