Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Induction of Protein Deletion Through In Utero…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Journal (Developmental Biology)
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Journal (Developmental Biology)
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
 

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Article DOI: 10.3791/51983-v • 12:01 min • January 12th, 2015
January 12th, 2015 •
1, 1, 1, 1
1Department of Cellular and Molecular Medicine, University of Ottawa

Chapters

Summary
Automatic Translation

Neuron migration is regulated by numerous cell autonomous and non-cell autonomous factors. This protocol shows how in utero electroporation can be used to determine whether a phenotype in a transgenic mouse is due to disruption of a cell intrinsic mechanism or impairment of interaction between the neuron and its environment.

Tags

Protein Deletion In Utero Electroporation Transgenic Models Neuronal Migration Cre-Lox System Phenotypes Cell Autonomous Mechanism Non-cell Autonomous Mechanism In Vivo Context Small Subset Of Cells Developing Cortex Brain Region Dorsal Telencephalon Medial Telencephalon Hippocampus Ganglionic Eminence Radial Migration Floxed Transgenic Mouse Line
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter