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JoVE Journal
Immunology and Infection

This content is Open Access.

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
 

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Article DOI: 10.3791/52159-v 08:27 min March 24th, 2015
March 24th, 2015

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Summary

Intact class I HLA/peptide complexes are shed by cancer cells, representing a potential relevant cancer biomarker. Utilizing label-free sensor technology and T-cell receptor mimicking monoclonal antibodies, detection of shed MIF/HLA-A*02:01 complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma is demonstrated, enabling development of a novel cancer diagnostic platform.

Tags

Human Leukocyte Antigen Biomarkers Breast Cancer Label-free Biosensor Technology T-cell Receptor Mimic Monoclonal Antibodies Cancer Diagnostics Companion Disease Diagnostics Personalized Medicine
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