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Journal / Bioengineering / Production and Targeting of Monovalent Quantum…
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Bioengineering

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JoVE Journal Bioengineering
Production and Targeting of Monovalent Quantum Dots
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Production and Targeting of Monovalent Quantum Dots

Article DOI: 10.3791/52198-v • 10:16 min • October 23rd, 2014
October 23rd, 2014 •
*1,2,3, *4,5,6, 1,4,7, 1,7, 4,5,6,7
1Department of Otolaryngology, University of California, San Francisco, 2Department of Chemistry, University of California, Berkeley, 3Materials Science Division, Lawrence Berkeley National Laboratory, 4Department of Pharmaceutical Chemistry, University of California, San Francisco, 5Tetrad Graduate Program, University of California, San Francisco, 6Center for Systems and Synthetic Biology, University of California, San Francisco, 7Chemistry and Chemical Biology Graduate Program, University of California, San Francisco
* These authors contributed equally

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Summary
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We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.

Tags

Production Targeting Monovalent Quantum Dots Multivalent Quantum Dots CdSe/ZnS Core/shell QDs Phosphorothioate DNA PtDNA Imaging Probes SNAP-tagged Notch Receptors Live Mammalian Cells Benzylguanine Moiety
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