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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Article DOI: 10.3791/52246-v • 13:47 min • February 24th, 2015
February 24th, 2015 •
*1, *1, 1, 1, 1,2, 1, 3, 4, 1
1Department of Medicine, Weill Cornell Medical College, 2Institute for Computational Biomedicine, Weill Cornell Medical College, 3Department of Physiology and Biophysics, Weill Cornell Medical College, 4Department of Pathology, University of Michigan
* These authors contributed equally

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Summary
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Enhanced Reduced Representation Bisulfite Sequencing is a method for the preparation of sequencing libraries for DNA methylation analysis based on restriction enzyme digestion combined with cytosine bisulfite conversion. This protocol requires 50 ng of starting material and yields base pair resolution data at GC-rich genomic regions.

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Enhanced Reduced Representation Bisulfite Sequencing DNA Methylation Base Pair Resolution Cytosine Methylation GC-rich Genomic Loci Restriction Enzyme Bisulfite Conversion Library Preparation Next Generation Sequencing Amplification Quantitative Base Resolution Protocol Sequencing Control Lane Mapping Bioinformatics Analysis
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