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Bile Duct Ligation in Mice
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Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Article DOI: 10.3791/52438-v • 08:56 min • February 10th, 2015
February 10th, 2015 •
1, 1, 1, 1, 2, 3, 1
1Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, RWTH Aachen University, 2Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, 3Department of Medicine III, RWTH Aachen University

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Summary
Automatic Translation

Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.

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Bile Duct Ligation Mice Inflammatory Liver Injury Fibrosis Obstructive Cholestasis Extrahepatic Biliary System Cholestasis Surgical Ligation Common Bile Duct Obstructive Cholestatic Injury Molecular Events Cellular Events Surgical Techniques Reconnection Reanastomosis Partial BDL Microsurgical Methods Complete Obstruction Fibrotic Response Mortality Rate Infectious Complications Technical Inaccuracies
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