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Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
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Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Article DOI: 10.3791/52522-v • 11:41 min • March 10th, 2015
March 10th, 2015 •
1, 2, 3, 4, 5
1USGS National Wildlife Health Center, 2Department of Soil Science, University of Wisconsin–Madison, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4Merial Veterinary Scholars Program, School of Veterinary Medicine, University of Wisconsin–Madison, 5Department of Neurology, University of British Columbia

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Summary
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Measuring the barrier to the interspecies transmission of prion diseases is challenging and typically involves animal challenges or biochemical assays. Here, we present an in vitro prion protein conversion assay with the ability to predict species barriers.

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Transmissible Spongiform Encephalopathy TSE Prion Diseases In Vitro Assay Prion Protein Conversion Conversion Efficiency Ratio CER Assay PH 7.4 Substrate PH 3.5 Substrate Proteinase K (PK)-resistant State Species Barrier Laboratory Mice Bobcats White-tailed Deer Chronic Wasting Disease Agent
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