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Immunology and Infection

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JoVE Journal Immunology and Infection
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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Article DOI: 10.3791/52589-v • 05:58 min • April 20th, 2016
April 20th, 2016 •
1,2, 1, 1,2,3, 1,3
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, 2Boston Children's Hospital, Division of Gastroenterology, Harvard Medical School, 3Humanitas Clinical and Research Center

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Summary
Automatic Translation

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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Single-cell Suspensions Cytofluorimetric Analysis Mouse Skin Regions Resident Immune Cells Cell Surface Markers Skin Samples Mouse Ear Forceps Cartilage Chunk Skin Samples Clippers Depilatory Lotion Vertical Cut Back Muscles Peritoneum Subcutaneous Fat Tail Skin Sample Surgical Scissors
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