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Journal / Developmental Biology / Immunostaining to Visualize Murine Enteric…
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JoVE Journal Developmental Biology
Immunostaining to Visualize Murine Enteric Nervous System Development
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Immunostaining to Visualize Murine Enteric Nervous System Development

Article DOI: 10.3791/52716-v • 07:54 min • April 29th, 2015
April 29th, 2015 •
1, 1, 2, 1,2,3
1Department of Surgery, University of Wisconsin School of Medicine and Public Health, 2Department of Neurosciences, University of Wisconsin School of Medicine and Public Health, 3Department of Pediatrics, University of Wisconsin School of Medicine and Public Health

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Summary
Automatic Translation

The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.

Tags

Immunostaining Visualize Murine Enteric Nervous System Development Neural Crest Cells Gut Colonization Differentiate Cholinergic Neurons Neurotransmitter Phenotype Staining Choline Acetyltransferase (ChAT) Acetylcholine Synthesis Antibody Specificity Central Nervous System Peripheral Nervous System Placental ChAT Antibody Embryonic Enteric Cholinergic Neurons Genetic Reporters For ChAT Reliability Technique Dissecting Fixing Immunostaining Murine Embryonic Gastrointestinal Tract Enteric Nervous System Neurotransmitter Expression
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