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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Article DOI: 10.3791/52975-v • 14:09 min • August 4th, 2015
August 4th, 2015 •
*1, *2, *3, *1, 4,5, 6, 1
1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University
* These authors contributed equally

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Summary
Automatic Translation

We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.

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Fluorescence Biomembrane Force Probe Receptor-ligand Kinetics Binding-induced Intracellular Signaling Single Cell Mechanical Environment Antigen Recognition T-cell Receptor Fluorescence Biomembrane Force Probe (fBFP) Single-molecule Force Spectroscopy Fluorescence Microscopy Force Sensor High-speed Camera Real-time Imaging Tracking Techniques Binding-triggered Intracellular Calcium Signaling Live Cell Membrane Receptor-ligand Interaction
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