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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Article DOI: 10.3791/53069-v • 08:47 min • March 30th, 2016
March 30th, 2016 •
*1, *1, 1, 2, 1, 1
1Department of General-, Visceral- and Transplantation Surgery, Charité University Medicine Berlin, 2Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin
* These authors contributed equally

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Summary
Automatic Translation

A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.

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Primary Human Hepatocytes Non-parenchymal Liver Cells Hepatology Toxicology In Vitro Co-culture Tissue Engineered Liver Models Liver Cell Populations Liver Tissue Laminar Airflow Hood Perfusion Solution Cannulas Tissue Glue Buchner Funnel Peristaltic Pump Flow Rate Blood Vessels
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