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Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule
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Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule

Article DOI: 10.3791/53144-v • 13:30 min • November 25th, 2015
November 25th, 2015 •
1, 1
1Department of Internal Medicine, University of Texas Southwestern Medical Center

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This protocol describes the use of the Ramsay assay to measure fluid secretion rates from isolated Malpighian (renal) tubules from Drosophila melanogaster. In addition, the use of ion-specific electrodes to measure sodium and potassium concentrations in the secreted fluid, allowing calculation of transepithelial ion flux, is described.

Tags

Ramsay Assay Fluid Secretion Ion Flux Rates Drosophila Melanogaster Malpighian Tubule Renal Epithelial Ion Transport Molecular Mechanisms Ion-specific Electrodes Sodium Concentrations Potassium Concentrations Transepithelial Fluid Fluxes Tubule Analysis Specific Genes In Transport Processes Bathing Saline Modification Chemical Characteristics Drugs Hormones
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