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JoVE Journal Bioengineering
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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Article DOI: 10.3791/53175-v • 10:30 min • September 2nd, 2015
September 2nd, 2015 •
1, 1, 1
1Biochemistry and Biophysics, Texas A&M University

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Summary
Automatic Translation

We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.

Tags

Macromolecular Delivery Cellular Entry Endosomal Entrapment DfTAT Reagent Cell-penetrating Peptide (CPP) Cytosolic Delivery Primary Cells Cell Viability Proliferation Gene Expression Small Molecules Peptides Antibodies Enzymes Transcription Factor DfTAT Synthesis Cellular Delivery Protocols Protein Administration Protein Delivery Control Technology Limitations Delivery Validation Cell-based Assays
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