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Journal / Biology / Production of Double-stranded DNA Ministrings
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Biology

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JoVE Journal Biology
Production of Double-stranded DNA Ministrings
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Production of Double-stranded DNA Ministrings

Article DOI: 10.3791/53177-v • 06:12 min • February 29th, 2016
February 29th, 2016 •
1, 1, 1, 2, 1
1School of Pharmacy, University of Waterloo, 2Sgd Health Innovation

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Summary
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The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.

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DNA Ministrings Gene Delivery DNA Vector In Vivo Approach LB Agar Ampicillin Media Preparation Overnight Culture W3NN PDMS Cells
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