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JoVE Journal Bioengineering
In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling
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In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

Article DOI: 10.3791/53224-v • 07:30 min • November 3rd, 2015
November 3rd, 2015 •
1, 2, 1
1Department of Mechanical Engineering, University of Colorado at Boulder, 2Product Development, Covidien, Ltd.

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This protocol replicates physiological or pathological blood flow in vitro to aid in determining cell response in disease pathologies. By introducing a pressure damping chamber downstream of a blood pump, blood flow across the vasculature can be recapitulated and imposed on a monolayer of vascular endothelium or a mimetic co-culture.

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In Vitro Model Physiological Blood Flow Pathological Blood Flow Vascular Cell Remodeling Vascular Disease Flow Dynamics Wall Stiffening Scarring Partial Stenosis Fluid Flow Rates Pulsatile Flow Pulsatility Index Flow Pressure Damping Chamber Blood Pump Compressible Medium Pulsatile Pressure Approximated Pulse Waveform Endothelial Cells Smooth Muscle Cells Fibroblasts Cardiac Output Arterial Wall Compliance Mechano-transduction Cytokine Release Cell-cell Interaction
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