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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
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Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Article DOI: 10.3791/53225-v • 09:32 min • November 11th, 2015
November 11th, 2015 •
1, 2, 1, 1, 1
1Department of Cardiology, Keio University School of Medicine, 2Department of Emergency and Critical Care Medicine, Keio University School of Medicine

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Summary
Automatic Translation

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

Tags

Induced Pluripotent Stem Cells Peripheral T Cells Sendai Virus Feeder-free Conditions Regenerative Therapies Dermal Fibroblasts Retroviral Genomic Insertion Antigen-specific T Cells T Cell Receptor (TCR) Genes Clinical Application Fetal Bovine Serum Pluripotent Stem Cell Culture Pathogenicity Protocol Undefined Pathogens Biosafety Level
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