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Immunology and Infection

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JoVE Journal Immunology and Infection
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
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Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Article DOI: 10.3791/53374-v • 08:09 min • November 16th, 2015
November 16th, 2015 •
1, 2, 2, 1, 1, 1
1Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, 2Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset

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Summary
Automatic Translation

Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.

Tags

Isolation Protocol B Lymphocytes T Lymphocytes Human Palatine Tonsils Immune System First Line Of Defense Tonsils Palatine Tonsils Oral Part Of The Pharynx Surgically Removed Accessible Source Interplay Between Pathogens And Immune System Video Protocol Dissection And Processing Individual B And T Cell Populations Antibody-dependent Affinity Protocol Human Adenovirus Tonsillar Immune Responses
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